Easy protocol—mix and spin
Scalable to any volume or titer
100X concentration with 90% recovery
The Lenti-X gravity column-based method is extremely effective, and preserves virus much better than syringe filter-based methods. Just bind, wash, and elute.
Gentle gravity column-based protocol
Does not damage lentiviral particles
Results in better transduction efficiency
Concentrates virus up to 10-fold
Add Lenti-X Concentrator reagent to clarified viral supernatant, incubate for 30 min at 4ºC, and spin.