Apply 20 μl of your lentiviral supernatant to the sample well, add 4 drops of buffer, and wait for the indicator bands to appear. Two bands mean you have ≥ 5 × 105 IFU/ml.
Lentiviral supernatant is bound to anti-p24 coated wells and detected using a combination of biotinylated anti-p24 secondary antibody, streptavidin-HRP, and a development reagent.
Set up the qRT-PCR reaction with purified RNA from harvested lentiviral supernatant. Determine the viral genome content from a calibrated RNA standard curve.
Quantify the number of lentiviruses that have integrated into the nuclear DNA of your target cells using a SYBR Green-based qPCR reaction.
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