Magnetic bead-based—no polybrene
For lentivirus & MMLV retrovirus
Ideal for sensitive cell types (e.g. stem cells)
Transduction time of 5 min
Use RetroNectin-coated tissue culture plates to enhance lentivirus-mediated gene transduction into mammalian cells. The
multivalent RetroNectin peptide allows for simultaneous binding of cells and lentivirus (LV), bringing them into close physical proximity. The RGDS-containing domain (green) binds the cell surface integrin receptor VLA-5, the heparin-binding domain binds many types of virus particles, and the CS-1 sequence (orange) binds the VLA-4 cellular integrin receptor.
Multivalent molecule simultaneously binds virus particles and cell surface proteins
Widely used for hematopoietic cells and stem cells
Increased transduction of hard-to-infect cells
With the Lenti-X Accelerator, a magnetic field concentrates virus-bound magnetic beads at the cell monolayer surface. This reduces the exposure time of sensitive target cells to viral supernatant to just 5 min—compared to overnight transduction with polybrene. The Lenti-X Accelerator starter kit includes a magnetic plate.