Your questions about site-directed mutagenesis…answered!

Read our application note for tips and tricks on how to use In-Fusion® HD Cloning Plus to perform site-directed mutagenesis without sacrificing efficiency.

  • You’ll learn how to use In-Fusion PCR Cloning to perform deletions, base substitutions, or insertions.
  • Timeline shows easy-to-follow steps from vector selection to final construct.
  • Protocol overview included to help you plan your next mutagenesis study.

Getting over 95% efficiency in site-directed mutagenesis?

Figure 1. Procedure for performing mutagenesis with In-Fusion systems. The area where mutagenesis occurs is shown in yellow.

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